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Happy Herping!
hi all..
my research make me confused. little bit. heheh. so, if you don’t mind, i want to discussing my research in here..
in my reference about my research, i get this:
“Lethal toxicity: The median lethal dose (LD50) of cobra
and krait venom was determined according to the
method developed by Theakston and Reid 1983.
Various doses of venom in 0.2 mL of physiological
saline was injected into the tail vein of mice (18-20 g),
using groups of 3-5 mice at each venom dose. The LD50
was calculated with the confidence limit at 50%
probability by the analysis of deaths occurring within
24 hours of venom injection. The anti-lethal potentials
of M. pudica plant extracts were determined against
2LD50 of cobra and krait venom. Various amount of
plant extracts (µL) were mixed with 2LD50 of venom
sample and incubated at 37°C for 30 min and then
injected intravenously into mice. 3-5 mice were used at
each antivenom dose. Control mice received same
amount of venom without antivenom (plant extracts).
The median effective dose (ED50) calculated from the
number of deaths within 24 hours of injection of the
venom/antivenom mixture. The ED50 was expressed
as µL antivenom/mouse and calculated by probit
analysis.”
my questions:
1. can u help me which method developed by Theakston and Reid 1983 ?
2. in the ED50 venom/antivenom was injected into the mice. i think, if we want to injected subtance, it must be sterile. am i wrong? can you explain me?
thanks..
i’m sorry if my english really bad.
i’m waiting for your answer..
Looks like there are a couple papers by Theakston and Reid from 1983. I came across these two that are open access and free. See if they help:
Yes, anytime you are injecting or performing medical procedures you want all your equipment to be sterile. If it’s not sterile you could accidentally introduce a bacteria or virus or such that may give you false readings and results plus it compromises the health of your study subject.
Saw a snake in Perdido Bay (costal Alabama) around noon today, May 16, swimming
ashore. About 18″ protruding out of the water. It got ashore and lay in the sun for
a while. Eight feet long, about 3-5″ max diameter. Seemed slender compared with
snakes I’ve seen of similar length. Head did not appear to be diamond shaped. Most
distinctive feature was its coloring — very dark near the nose & eyes, grew progressively
lighter to about 2 feet back. Remainder of the body was quite light — white. I’ve been
coming to this area for 60 years and have never seen such a thing. Can you identify?
Possibly a captive snake a boater threw over? Unfortunately, the snake disappeared
while I went to retrieve camera. I was able to observe it very well for a minute or two
from about five feet from its head.
Lee,
That’s definitely an odd sighting along the coast of Alabama to say the least. The only US snake that comes close to 8′ long is the eastern indigo snake and it’s blackish blue and not marine. I’m not sure what species you saw but it doesn’t sound like a native one.